芘高效降解菌的分离鉴定及其降解特性

Ming Zhong,Jia Qing Zhang,XiaoXia Wu,YANG Feng,MA Hui,CHEN Li-jing

Chinese Journal of Applied Ecology（2010）

Cited 4|Views14

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Abstract

以芘为唯一碳源,采用富集培养方法,从沈抚灌区石油污染土壤中分离得到一株芘降解菌ZQ5.根据形态学观察、生理生化鉴定和16S rDNA序列分析结果,将菌株ZQ5鉴定为寡养单胞菌属(Stenotrophomonas sp.).采用摇瓶振荡培养方法研究该菌株降解芘的特性及培养条件对降解效能的影响.结果表明:菌株ZQ5在30℃振荡培养10d后,对100mg·L-1的芘降解率为91.2%,加入水杨酸(100mg·L-1)作为共代谢底物可以提高菌株ZQ5对芘的降解率.当培养基pH为7～8、盐浓度不高于2%时,有利于菌株ZQ5降解效能的发挥.

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Key words

bacterial strain,degrading characteristics,high-efficient,pyrene-degrading

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