Impact of survivin-targeted RNAi on growth and apoptosis of implanted human cervical carcinoma in mice

Objective: To observe the effect of RNAi targeting survivin gene(RNAi-survivin) on the growth and apoptosis of implanted human cervical carcinoma in mice.Methods: Nude mice were randomly divided into 4 groups,and the human cervical carcinoma HeLa cells transfected with pSilencer2.1-s2,pSilencer2.1-NC,pSilencer2.1-U6 neo or not transfected(the cells being HeLa-s2,HeLa-NC,HeLa-U6 neo and Hela,respectively) were subcutaneously inoculated into flank tissues of mice to establish cervical carcinoma mouse models.The influence of survivin-RNAi on the implanted tumor growth was observed.Expression of survivin protein and microvessel density(MVD) in implanted cervical carcinoma tissues were examined by immunohistochemistry.Apoptosis of implanted cervical carcinoma cells was observed by H-E staining and TUNEL.Results: Implanted human cervical carcinoma mouse model was successively established.The tumor weight in HeLa-s2 group was markedly lower than those in HeLa-NC,HeLa-U6 neo and Hela groups(P0.05).The tumor inhibitory rate in HeLa-s2 group was 67.9%.Survivin expression and MVD value in tumor tissues of HeLa-s2 group were significantly decreased as determined by immunohistochemistry(all P0.05).Apoptosis of implanted cervical carcinoma cells in HeLa-s2 group was significantly increased compared with those in other 3 groups(all P0.05),with AI being(22.73±1.37)%.Conclusion: RNAi-survivin inhibits the growth and promote apoptosis of implanted human cervical carcinoma tumors by down-regulating survivin protein expression and MVD in tumor tissues.