Analysis of different expression of CDPKs genes in the dedifferentiation of mature wheat embryo
Plant Physiology Communications(2009)
Abstract
Expression profiles of CDPKs genes were analyzed by Affymetrix Microarray technique during wheat mature embryo dedifferentiation. Out of 16 CDPKs genes detected by microarray, 15 genes were significantly changed in expression pattern during dedifferentiation of mature wheat embryo. Furthermore, 12 genes were up-regulated by 2-7.5 times, 2 genes were down-regulated by 3-6.1 times, and 1 gene was up- or down-regulated at the different stages of dedifferentiation. According to the known functions of CDPKs, their changed trend and the coincidence of the trend and the dedifferentiation degree, it was predicted that CPK2A, CPK2B, CPK6 and Osl2g0169800 might play roles in the initiation of dedifferentiation of mature wheat embryos and the formation of calli.
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Key words
CDPKs,Dedifferentiation,Gene microarray,Protein kinases
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