cDNA cloning and analysis of flavonoid 3′-hydroxylase (F3′H) in Vitis amurensis Rupr.

The full-length cDNA sequences of F3’H genes in Vitis amurensis were cloned by the technique of RT-PCR and SMART RACE. The landing Genbank No. is FJ645766 and the full-length of F3’H cDNA is 1 844 bp with open reading frame of 1 530 bp, which encodes 509 amino acids. The deduced amino acid of F3’H gene was unstable protein with the molecular mass of 56.14 kDa and isoelectric point of 8.24. The F3’H gene belongs to the P450 supergene family, without a signal peptide. The amino acid sequence of F3’H in Vitis amurensis had the homology with F3’H of Vitis vinifera (AJ8803537), Centaurea cyanus (FJ753550), Antirrhi- num majus (DQ272592), Glycine max (AB061212) and Zea mays (EU966228), and the homology coefficient was 98%, 77%, 72%, 74% and 57% respectively.