石蜡半薄切片制作透射电镜的方法

Journal of Dalian Medical University(2009)

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Abstract
[目的]介绍石蜡半薄切片转制透射电镜的制样方法.[方法]对石蜡半薄切片进行脱蜡、水化、锇酸固定、缓冲液漂洗、梯度酒精脱水、环氧树脂包埋、超薄切片机切片、透射电镜下观察.[结果]组织细胞的超微结构保存完好,足以满足病理诊断之用.[结论]用石蜡半薄切片转制电镜比用石蜡组织块转制电镜方法简单,快速易行.
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Key words
Electron microscopy sample production,Semi-thin paraffin sections,Transmission electron microscopy
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