Histone deacetylase inhibitor trichostatin A modulates cell cycles in A2780 human ovarian cancer cell lines

Purpose of investigation: To analyze the effect and mechanism of TSA on cell cycles in human ovarian cancer cells. Methods: cells were cultured in RPMI 1640 supplemented. Flow cytometry analysis and RT-PCR were used to examine the distribution of cell cycles and the level of P21(WAF/CIPI) mRNA. Results: TSA induced increase of the G(2)/M phase accompanied by decrease of the S phase and enhanced level of P21(WAF/CIPI) mRNA in a concentration and time-dependent manner in A2780 cells. Conclusion: Trichostatin A affects the activity of cyclin-dependent kinase through increased expression of P21(WAF/CIPI) mRNA. TSA causes A2780 cell blockage in the G(2)/M phase and inhibits cell proliferation of A2780 cells. The minimum level of active TSA is 100nM and the minimum time is 12 hours. The effect relies on time and concentration.