Identification of human leukocyte antigen-DRB1*1454 allele and human leukocyte antigen-DRB1 exon 3 sequence information in the Chinese population

Aim: To identify human leukocyte antigen (HLA)-DRB1*1454 allele and HLA-DRB1 exon 3 sequence information in the Chinese population, which is significant for organ transplantation, cell transplantation, and human genetics. Methods: Polymerase chain reaction sequence-based typing (PCR-SBT) was used to identify HLA-DRB1 alleles from 58 donor-recipient individuals who would undergo haemopoietic stem cell transplantation. Medium to high resolution polymerase chain reaction-reverse sequence specific oligonucleotide probe (PCR-RSSOP) was used to identify HLA-DRB1 alleles from 1 268 healthy donors from Guangdong province. The some ambiguous results of HLA-DRB1*14-associated alleles were confirmed by high resolution polymerase chain reaction-sequence-specific primer typing (PCR-SSP). Results: HLA-DRB1*1403, 1406, 1410, 1412, 1418, 1425 and 1454 alleles were detected in 1 268 healthy donors. HLA-DRB1*1454 was confirmed in 8 ambiguous results of HLA-DRB1*1401/1434/1454 alleles, and HLA-DRB1*1454 was one of common alleles of HLA-DRB1*14 allele group in Guangdong population. HLA-DRB1*14 exon 3 sequence information was confirmed to be polymorphic in Chinese population. Conclusion: HLA-DRB1*1454 and exon 3 of DRB1 are confirmed to be polymorphic in Chinese population, further elucidating that HLA-DRB1 exon 3 sequence information is important for Han population and some minority groups.