[Determination of monosaccharides in honey and polysaccharide hydrolyzate of functional food by high performance anion exchange chromatography].

OBJECTIVE:To develop a method for analysis of eight kinds of monosaccharides using high performance anion exchange chromatography. METHODS:Amino PAC PA-10 (2 x 250 mm) column with a pulse amperometric detector with an Au working electrode and an Ag/AgCl reference electrode was applied to determine the monosaccharides. RESULTS:The linear range was 0.1-5.0 microg/mL and the detection limits were 2-26 microg/L. The relative standard derivation for retain time and area of standard solution were 0.34%-7.42% and 0.02%-2.16% respectively. The satisfactory results were obtained for analysis of honey and polysaccharide hydrolyzate of functional food with the proposed method. The average recoveries were 81.4%-122.0%. The method was sensitive and with high precision. CONCLUSION:This method was available for determination of monosaccharides in honey and polysaccharide hydrolyzate of functional food.