Research on optimization system on tissue culture and rapid propagation of Pueraria mirifica

Objective: To study the tissue culture and rapid-proliferation techniques of Pueraria mirifica. Method: The tender branch were used as explants and cultivated in different media. The optimum media for inducing buds, proliferation and rooting were selected by adjusting the kinds and doses of plant hormones and special compounds. Result: The medium of MS + IBA 0.05 mg·L -1 + BA 0.5 mg·L -1 was suitable for buds' inducing and could be used in the first generation cultivation; MS + IBA 0.02 mg·L -1 + BA 0.2 mg·L -1 and MS + BA0.1 mg·L -1 were employed by turns in subculture, 25 days propagation coefficient was 3.0; and the medium of 1/2MS + IBA 0.1 mg·L -1 + IAA 0.2 mg·L -1 + C (special compound) 10 mg·L -1 was used for roots inducing, the rooting rate was 76.9%. Rooting plantlets were transplanted in spring and summer; the surviving rate was 81.0%. Conclusion: This technique system could be employed for rapid propagation of P. mirifica.