Production of recombinant human interferon α2b in batch and fed-batch culture of Escherichia coli BL21 (pBAI)

E. coli BL21 (pBAI) was used for the production of hIFNα2b whose expression was under the control of PL promoter. Batch and fed-batch cultures for hIFNα2b production in complex media were compared at the induction temperature of 42°C. By feeding glucose at a constant rate, hIFNα2b production reached 6540 mg/L, with an overall productivity of 546 mg/(L·h) and an overall specific productivity of 27 mg/(g·h). By supplying yeast extract before induction and feeding glucose at a specific provision rate of 0.27 g/(g·h), the overall productivity was as high as 1006 mg/(L·h), and the specific productivity reached 54 mg/(g·h) with an overall yield of 138 mg/g based on total complex nitrogen sources.