LC-MS Determination and Pharmacokinetic Study of Luteolin-7- O -β- d -glucoside in Rat Plasma after Administration of the Traditional Chinese Medicinal Preparation Kudiezi Injection

A rapid and sensitive LC-MS method has been developed for the determination of luteolin-7- O -β- d -glucoside in rat plasma after solvent extraction. Separation was on an Elite Hypersil ODS2 column (250 mm × 4.6 mm i.d., 5 μm) with a mobile phase of acetonitrile-0.3% acetic acid (26:74, v/v ). The samples were analyzed by using positive electrospray ionization MS in selected ion monitoring mode. The selected ions for luteolin-7- O -β- d -glucoside and the internal standard, isoquercitrin, were m/z 448.95 and m/z 464.95. Good linearity was observed over the range of 20–2,000 ng mL −1 with a lower limit of quantification of 20 ng mL −1 . No interference peaks or matrix effects were observed. The validated method was applied to the pharmacokinetic study of luteolin-7- O -β- d -glucoside in rat plasma after intravenous administration of Kudiezi Injection.