植物雌激素金雀异黄酮通过p38MAPK通路促进骨髓间充质干细胞向成骨细胞分化
Chinese Pharmacological Bulletin（2006）
Abstract
目的研究丝裂原激活的蛋白激酶（m itogen-activatedprote in k inases,MAPKs）的亚型p38 MAPK在植物雌激素金雀异黄酮（Gen iste in）促进小鼠骨髓间充质干细胞（bone m ar-row-derived m esenchym al stem cells,BMSCs）向成骨细胞分化过程中的作用。方法BMSCs用无酚红-αMEM（含经活性碳吸附的10%FBS、β-磷酸甘油、维生素C）培养,先用Gen iste in处理细胞,观察BMSCs向成骨细胞分化情况,然后用SB203580（p38 MAPK通路阻断剂）以及PD98059（p44/42MAPK通路阻断剂）阻断相应的通路后,再用Gen iste in处理细胞,观察BMSCs向成骨细胞分化情况,并同时观察上述处理后MAPK通路的变化。测定碱性磷酸酶（ALP）活性和钙（Ca）沉积量反映BMSCs向成骨细胞分化状况,用W esternb lot来检测MAPK通路是否激活。结果Gen iste in（0.01,0.1,1μmol.L-1）剂量依赖性增加小鼠BMSCs细胞内ALP活性和细胞外Ca沉积量,并同时引起p38MAPK通路的激活和p44/42MAPK通路的抑制。SB203580预处理能减弱Gen iste in刺激引起的p38MAPK通路的激活并同时阻止Gen iste in诱导的BMSCs向成骨细胞分化。结论Gen iste in在0.011μmol.L-1剂量范围内可通过p38MAPK通路促进小鼠BMSCs向成骨细胞分化。
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Key words
Bone marrow-derived mesenchymal stem cells (BMSCs),Genistein,Mitogen-activated protein kinases (MAPKs),Osteoblastic differentiation
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