肝素-ELISA法检测血清中期因子及其对肝癌诊断的初步应用
Academic Journal of Second Military Medical University(2006)
Abstract
目的:建立中期因子(midkine,MK)的测定方法,初步探讨血清MK浓度测定对肝癌的诊断价值.方法:根据MK可结合肝素的特点,应用肝素-抗体双夹心原理,建立肝素-ELISA,并检测20例健康献血者、20例良性肝占位性病变患者、20例肝硬化患者以及104例肝细胞癌(HCC)患者血清MK的质量浓度.结果:肝素-ELISA检测MK的最小值为15 ng/L,可测范围为15~1 000 ng/L,<500 ng/L时呈线性关系(r=0.97).测定结果表明,正常献血者、良性肝占位性病变患者和肝硬化患者血清MK浓度分别为(56±11)、(74±19)和(89±43)ng/L,而HCC患者血清MK浓度为(199±87)ng/L,与前3组之间有显著差异(P<0.05).HCC患者血清MK阳性率达83.7%,与其他3组之间具有显著性差异(P<0.001).结论:应用肝素-ELISA测定血清MK的质量浓度对肝癌具有诊断价值.
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Key words
Enzyme linked immunosorbent assay,Heparin,Hepatocellular carcinoma,Midkine
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