Real-time polymerase chain reaction for detecting SARS coronavirus, Beijing, 2003.

Junhui Zhai,Thomas Briese,Erhei Dai,Xiaoyi Wang,Xin pang,Zongmin Du,Haihong Liu,Jin Wang,Hongxia Wang,Zhaobiao Guo,Zeliang Chen,Lingxiao Jiang,Dongsheng Zhou,Yanping Han,Omar Jabado,Gustavo Palacios,W Ian Lipkin, Ruifu Tang

EMERGING INFECTIOUS DISEASES（2004）

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摘要

During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3' end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to screen >700 clinical samples.

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bioinformatics,epidemiology,biomedical research,virology

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