荧光素共振光散射法测定脱氧核糖核酸
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY（2004）
Abstract
研究了咕吨类染料荧光素Fluorescein(FL)与DNA作用的共振光散射光谱.加入DNA后,在pH 6～8的范围内,荧光素在DNA分子表面发生长距离自组装,在400 nm处产生了增强的共振光散射峰,其发光强度与DNA的浓度呈线性关系,线性范围为0.04～3.1 mg/L; 检出限为16 μg/L.考察了影响因素和最佳反应条件,建立了用RLS光谱测定ng级DNA的新方法.
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Key words
Deoxyribonucleic acid,Fluorescein,Resonance light-scattering
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