HPV-6/11 L1/E6、HPV-6/11 L1/E7嵌合DNA疫苗质粒的构建
Journal of Xi'an Jiaotong University (Medical Sciences)（2003）
Abstract
目的构建HPV-6/11 L1/E6、HPV-6/11 L1/E7预防、治疗性DNA疫苗质粒.方法运用PCR扩增HPV-6/11 L1、E6、E7序列,PCR产物连接至pGEM T-Easy质粒,测序鉴定正确后,分别连接至真核表达载体pVAX,再用酶切、连接而构建成HPV-6/11 L1-E6/E7-pVAX质粒.运用电穿孔法将所获得质粒转染COS-7细胞,免疫组化检测蛋白表达情况.结果所构建质粒的插入目的片段测序正确;免疫组化检测在胞浆胞核可见棕黄色点状阳性产物沉积.结论所构建的HPV-6/11 L1-E6/E7-pVAX融合蛋白表达质粒可在体外表达L1-E6/L1-E7蛋白,为今后进行DNA疫苗的动物实验及临床实验研究做好准备.
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Key words
Cervical cancer,DNA vaccine,HPV-6/11
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