Genotyping and distribution of mutant CCR5Δ32, CCR2-641 and SDF1-3′ A alleles in HIV-1 carriers and patients with sexually-transmitted diseases and chronic HBV infection

AIM: To evaluate the polymorphisms of mutant CCR5Δ32, CCR2-641 and SDF1-3′ A alleles in HIV-1 carriers and patients with sexually-transmitted disease (STD) and chronic HBV infection from Han ethnic population in China. METHODS: The genomic DNAs from all 657 patients were purified from whole peripheral blood samples of HIV-1 carriers (n = 130), patients with (n = 259) and the patients with chronic HBV infection (n = 268) using QIAamp DNA Blood Mini Kit. The genotyping and distribution of CCR5Δ32, CCR2-641 and SDF1-3′ A alleles were further identified by means of PCR or PCR-RFLP assays and statistical analyses. RESULTS: The mutation frequency of CCR5Δ32 alleles was 0%, 0. 19% and 0% for HIV-1 carriers, STD and HBV-infected patients, respectively, which is similar to the features with a very low frequency of CCR5Δ32 mutant in healthy individuals from Chinese Han population as previously reported. Comparatively, there was a higher frequency of CCR2-641 genes of 19.62%, 19.31% and 20. 7%, and of SDF1-3′ A alleles of 27.8%, 27.4% and 26.9%, respectively, in the corresponding patients of aforementioned three groups. Statistical analyses showed that there are independent distributions of the three CCR5Δ32, CCR2-641 and SDF1-3′ A alleles in the HIV-1 carriers, HBV-infected patients and the STD patients. CONCLUSIONS: The evidence of very low frequency of mutant CCR5Δ32 allele suggested that this polymorphism is unlikely to play a major role as a host genetic factor, but the functions and its implications of higher frequency of CCR2-641 and SDF1-3′ A alleles remain to be further clarified in HIV-1 carriers as well as in STD cases and HBV-infected patients.