A Study On The Single- And Dual- Near-Infrared Fluorescent Labeled Nucleic Acid Conjugate For The Nucleic Acid Detection

In this research, a near-infrared fluorescent labeled nucleic acid conjugate for the nucleic acid detection was synthesized, and characterized preliminarily for the detection of the nucleic acid. The conjugate combines the molecular recognition properties of the oligonucleotides with the near-infrared fluorescent label PR646. Both single- and dual-labelled conjugates were studied for their hybridization with the complementary nucleic acid. The dual labelled conjugate has indicated that the self-quenchening effect exists in ssDNA form while the fluorescence increases greatly after hybridization with the complementary nucleic acids. The time-resolved fluorescence was also studied.