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应用激光共聚焦扫描技术对海马脑片神经元内钙的观察

Liu-hua Wang,PENG ZHAO,Ya-Lin HUANG,JIE-SHI CHENG

Sheng li xue bao : [Acta physiologica Sinica]（1998）

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Abstract
用微量注射法将荧光剂Fluo-3(AM)注入大鼠海马,对神经元进行在体荧光标记,可清晰地标记多个神经细胞.联合应用激光共聚焦扫描显微镜(confocallaser scanning microscope,CLSM),观察大鼠海马脑片CA1锥体细胞在青霉素、谷氨酸模拟致痫及缺糖缺氧时胞内钙的变化.结果显示:无镁时,谷氨酸和青霉素可致海马CA1锥体细胞胞内钙的缓慢增加;离体缺糖缺氧时CA1锥体细胞胞内钙亦增多.
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Key words
Confocal laser scanning microscopy,Fluo-3 (AM),Hippocampal slice,Intracellular calcium,Microinjection
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