Study on the correlations of P24 antigen, HIV DNA, HIV RNA in 139 HIV infected cases

Objective To study the relationship between the immune state of the host and P24 Ag, HIV DNA, HIV RNA and their inner relations. Methods We collected 139 anti -HIV seropositive blood samples to detect P24Ag, HIV DNA, HIV RNA by solidphase sandwichtype immunoassay(U. S. Abbott), nest PCR (Merke Germany) and RT-PCR(U. S. RocheBra), respectively, Results The number of HIV-DNA positive was 109/139 HIV RNA was 108/139, and P24Ag was 34/139, The further analysis:the rate of HIV DNA and HIV RNA both positive was 80%, 20% samples had only single positive reaction. Conclusions We consider that the sensitivity and correspondence for detecting HIV are close related to the gene mutation of HIV strains, variety of individual immunoregulation and some treatment factors. So it is important to use more HIV primers to test HIV DNA, RNA and P24Ag simultaneously. The results of the test must be analyzed with clinical symptoms. It will help us to understand the mechanism of HIV infection and the result of treatment for AIDS.