Fluorimetric determination of aesculin and aesculetine in frainus chinensis roxb

The fluorescence enhancement of aesculin and aesculetine by forming inclusion complexes with ��-cyclodextrin was utilized for the determination of their contents in frainus chinensis roxb. The linear range of determination, the detection limit and the average recovery of aesculin and aesculetine are 2��10-8��8��10-6 g/L and 2��10-9��8��10-6 g/L, 3. 78�� 10-10 and 5. 5��10-11 g/L, and 96. 6% and 96. 8%, respectively.