Morphine blocked the exitatory amino acid mediated membrane current in ambigual motoneurons of the rat

Excitatory postsynaptic potentials (EPSPs) and responses of neurons in the compact formation of the neucleus ambiguus (AMBc) to pressure-ejected or bath-applied test substances were recorded intracellularly from sagittal slices of Sprague-Dawley rat medulla containing subnucleus centralis of solitary complex (NTSc), AMBc and solitarioambigual pathway. In 5 cells, recorded spontaneous EPSPs could be blocked by morphine (3-5 pmol) to AMBc. Electrical stimulation of NTSc evoked EPSPs in AMBc neurons, the amplitude of which were decreased to 71.1��6.2% (P<0.001) with the addition to morphine. The morphine effect could be abolished by bath-applied naloxone (50 nmol/L). The amplitude of membrane depolarization induced by pressure-ejected N-methyl-D-aspartate (NMDA, 0.5-1.0 pmol), acetylcholine (3 pmol) and quisqualate (0.1-0.5 pmol) to NTSc could also be decreased by bath-applied morphine (10 ��mol/L) respectively to 38.1��5.7% (P<0.001), 32.8��5.5% (P< 0.01) and 29.6��7.1% (P<0.05). The above results suggest that morphine is capable of block by NMDA, ACh and non-NMDA receptors, increaseing the M-current and decreasing the permeability of Na+ and Ca2+.