Isolation and identification of bacteria capable of degrading euptox A from Eupatorium adenophorum Spreng.

Eupatorium adenophorum Spreng (E. adenophorum), an invasive plant which has caused widespread poisoning of livestock and crop failures in China. 9-oxo-10, 11-dehydroageraphorone (known as euptox A) is the main toxin causing a series of respiratory and digestive system diseases in animals. The purpose of the research is to isolate and identify the bacteria with the capacity of degrading euptox A, in which way could we lay the foundation in the utilization and prevention for E. adenophorum. Three strains of bacteria with the degradative characteristic are screened from E. adenophorum leaves, soil and goat's rumen respectively by nutrition enrichment. The 16S rDNA sequence of the three isolated strains (XC-07, XC-08, XC-09) were shown to be 97%, 100%, 100% similar to Stenotrophmonas sp., Klebsiella sp. and Pseudomonas sp., respectively, named as Stenotrophomonas sp. XC-07, Klebsiella sp. XC-08 and Pseudomonas sp. XC-09. The degradation rates of 3 strains to euptox A (45 mg/L) in 24 h are 91.2%, 94.3% and 93.2%, respectively, which are determined by HPLC.