Mutagenic Research on Ciliary Neurotrophic Factor (CNTF) in Escherichia coli After Heavy Ions Irradiation
Lecture Notes in Electrical Engineering(2014)
摘要
Based on the natural human CNTF gene, Ying et al. acquired CNTF-T mutant and constructed Escherichia coli expressing CNTF. In order to increase the yield and enhance the protein expression in the constructed E. coli, the strains were irradiated with high-LET heavy ions. Some mutants were obtained in this work and the mechanisms underlying the enhanced expression of CNTF in E. coli after exposure to heavy ions are discussed in this paper.
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关键词
High-LET irradiation, CNTF, Mutant, Molecular characterization
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