Expression Of Dectin-1 During Fungus Infection In Human Corneal Epithelial Cells

AIM: To evaluate the expression of dendritic cell associated C type lectin-1 (dectin-1) in human corneal epithelial (HCE) cells infected by fungus.METHODS: A total of 20 cases of healthy donor corneas were group A, and 20 patients (20 eyes) suffered from fungal keratitis (FK) composed group B. Real time qPCR and immunohistochemistry were applied to detect dectin -1 expression in corneal epithelium of both groups. HCE cells were cultured with aspergillus fumigatus (AF) antigens in lent The expression of dectin-1 mRNA was measured by real time qPCR at the stimulation of 0, 4, 8 and 24h separately. Dectin-1 protein was detected by immunocytochemistry at 0 and 24h separately.RESULTS: Dectin-1 expressed in corneal epithelium of normal persons and FK patients. Vitro cellular experiment showed that the expression of dectin -1 mRNA in HCE cells began to increase after stimulation of AF antigens at 4h, and dectin -1 protein expression increased after stimulation at 24h.CONCLUSION: Dectin -1 expressed in corneal epithelium of normal persons. AF antigens stimulation can elevate the expression of dectin -1 in HCE cells in vitro.