Promotion Of Mintbp-1-Prgdn On The Attachment, Proliferation And Collagen I Synthesis Of Human Keratocyte On Titanium

AIM: To investigate the influence of minTBP-1 PRGDN on the attachment, proliferation and collagen I synthesis of human keratocyte on titanium (Ti) surface.METHODS: The chimeric peptide RKLPDAPRGDN (minTBP -1 -PRGDN) was synthesized by connecting RKLPDA (minTBP-1) to the N-terminal of PRGDN, the influence of minTBP 1 PRGDN on the attachment, proliferation and collagen I synthesis of human keratocyte on Ti surface were tested using PRGDN and minTBP-1 as controls. The keratocytes attached to the surface of Ti were either stained with FITC labeled phalloidin and viewed with fluorescence microscope or quantified with alamar Blue method. The proliferation of keratocytes on Ti were quantified with 3 -(4,5 -dim - ethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide up-taking methods. The secretion of type I collagen was determined using an ELISA kit.RESULTS: The results showed that minTBP-1 PRGDN at a concentration of 100ng/mL was the most potent peptide to enhance the attachment of human keratocytes to the surface of Ti(1.40 +/- 0.03 folds, P=0.003), to promote the proliferation (1.26 +/- 0.05 folds, P=0.014) and the synthesis of type I collagen (1.530 +/- 0.128, P=0.008). MinTBP-1 at the same concentration could only promote the attachment (1.13 0.04 folds, P =0.020) and proliferation (1.15 +/- 0.06 folds, P=0.021), while PRGDN had no significant influence (P>0.05).CONCLUSION: Our data show that the novel chimeric peptide minTBP-1-PRGDN could promote the attachment, proliferation and type I collagen synthesis of human keratocytes on the surface of titanium.