Generation and evaluation of avian leukosis virus subgroup J envelope glycoprotein recombinant pseudovirions.

•Pseudovirions are a good model for studying the biology of ALV-J.•The envelope protein of ALV-J was integrated into the surface of pseudovirions for skeleton.•Pseudovirions were used to study the infectious tropism of ALV-J at the cellular level in vitro.•The antibody levels of ALV-J infections in Chickens could be rapidly detected.•The advantages of the traditional ELISA and MN were evaluated in detecting the level of antibody against gp85.