Purification And Characterization Of A Novel Alpha-Amylase From A Newly Isolated Bacillus Methylotrophicus Strain P11-2

•This is the first to report on the enzyme production by Bacillus methylotrophicus.•The α-amylase produced by strain P11-2 was purified to homogeneity.•The end products of enzyme reaction toward soluble starch are different from others.•The N-terminal sequence of the α-amylase showed no homology to other α-amylases.•Based on its characters, α-amylase of strain P11-2 should be a novel α-amylase.