Metabolic studies of temperature control strategy on poly(γ-glutamic acid) production in a thermophilic strain Bacillus subtilis GXA-28
Bioresource Technology(2014)
Abstract
•A thermophilic strain B. subtilis GXA-28 and its product were characterized.•γ-PGA yield reach 19.92g/L and molecular weight reach 3.03×106Da at 45°C.•MFA results indicate that temperature presents a marked impact on γ-PGA production.•Enzyme activity analysis shows that ICDH and GDH are the positive regulators.•Provide a potential strain and a temperature control strategy for γ-PGA production.
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Key words
Poly(γ-glutamic acid),Bacillus sutilis,Temperature,Metabolic flux,Enzyme activity
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