Effect of Hanfangji Compound on proliferation of hepatic stellate cells and expressions of TTR, ITIH1 and SERPINF2

Objective: To observe and compare the effects of Hanfangji Compound and IFN-γ on expressions of transthyretin (TTR), inter-α inhibitor H1 (ITIH1) and serpin peptidase inhibitor clade F member 2 (SERPINF2) of hepatic stellate cells (HSC-T6). Method: Hanfangji Compound and IFN-γ of different concentrations were used in hepatic stellate cell-T6 (HSC-T6) for 48 h. Flow cytometer was used to detect the effects of Hanfangji Compound and IFN-γ on HSC proliferation. RT-PCR method was adopted to detect mRNA expressions of TTR, ITIH1 and SERPINF2.TTR, ITIH1 and SERPINF2 secretions were detected by ELISA. The protein localizations of TTR, ITIH1 and SERPINF2 were examined by immune fluorescence. The protein expression of TTR and ITIH1 were determined by Western blot. Result: After Hanfangji Compound and IFN-γ were adopted in HSC-T6, compared with the control group, the cell proliferation was inhibited obviously (P < 0.05), protein expressions of TTR, ITIH1 and SERPINF2 and mRNA expression increased significantly, with certain correlation with concentrations of Hanfangji Compound. The 2.5 g·L-1 Hanfangji Compound group was superior to the IFN-γ group (P<0.05). Conclusion: Hanfangji Compound can inhibit HSC proliferation, up-regulated TTR, ITIH1 and SERPINF2 proteins and mRNA expression, which may be one of mechanisms of anti-hepatic fibrosis of Hanfangji Compound.