人参皂苷Rg1在造血干/祖细胞连续移植中对抗细胞衰老的作用研究
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica(2013)
Abstract
目的:探讨人参皂苷Rg1在造血干/祖细胞(HSC/HPC)连续移植中对抗细胞衰老的作用.方法:免疫磁性分选法分离纯化的雄性供体小鼠Sca-1+ HSC/HPC连续移植3代构建HSC/HPC衰老体内模型.60Co γ射线致死剂量辐射雌性受体鼠后分4组:对照组、衰老模型组、Rg1治疗衰老组、Rg1延缓衰老组.荧光定量PCR检测受体鼠骨髓细胞Y染色体(Sry基因)表达,确定受体鼠重建造血细胞来源;观察受体鼠存活时间及外周血血象指标的恢复,确定受体鼠造血功能重建情况及Rg1促进造血恢复情况;造血祖细胞混合性集落(CFU-Mix)培养,细胞周期分析和衰老相关β-半乳糖苷酶(SA-β-Gal)染色分析Sca-1+ HSC/HPC衰老的生物学特点及Rg1体内调控Sca-1+ HSC/HPC衰老的作用.结果:雌性受体鼠重建造血细胞来源于雄性供体鼠;连续移植后受体鼠30 d存活率明显降低,外周血象恢复延缓,Sca-1+ HSC/HPC出现细胞衰老特征:G0/G1期细胞比例及SA-β-Gal染色阳性率增高,CFU-Mix数量下降.与同代衰老模型组相比,Rg1治疗衰老组及Rg1延缓衰老组受体鼠30 d存活率,WBC,HCT,PLT增高,Sca-1+ HSC/HPC G0/G1期细胞比例、SA-β-Gal染色阳性率下降,CFU-Mix数量升高.Rg1延缓衰老组变化较Rg1治疗衰老组明显.结论:人参皂苷Rg1在HSC/HPC连续移植过程中具有延缓和治疗Sca-1+ HSC/HPC衰老的作用.Rg1延缓衰老作用优于Rg1治疗衰老作用.
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Key words
anti-aging,ginsenoside rg1,hsc/hpc,serial transplantation
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