Analysis of hepatitis B virus genotyping and drug resistance gene mutations based on massively parallel sequencing.

•Established a sensitive method for the detection of HBV drug resistance mutation and genotyping based on HiSeq-sequencing.•Compared with 3730 sequencing and MALDI-TOF mass spectrometry, HiSeq-sequencing has higher sensitivity in detecting the HBV gene mutation.•A mixed plasmid DNA of HBV was used to determine the cutoff value of 1%.