New integration technology on preparation of natural seleniferous rice protein peptide with enzyme and ultrasonic-microwave synergistic technology

The natural seleniferous rice protein peptide was prepared by coupling with mixed-enzymes and ultrasonic-microwave synergistic technology in this paper. The kinds of enzyme was atteined first of all. Then the mathematical model of correlation among enzyme dosage, extraction temperature, extraction time and liquid-solid ratio were established by means of orthogonal experimental design. The optimal extraction conditions were determined as follows, alkali protease dosage 2.0%, extraction temperature 50 degrees C, extraction time 40 min and liquid- solid ratio 7:1(m:g). Under such conditions, the extraction ratio was 72.2%, Se content was 6.88 mu g/ml. The method could significantly improve the yield of natural seleniferous rice protein peptide and reduce the extraction time.The rich selenium rice protein peptide provides a very good source of selenium way in order to solve the lack of selenium source.