Chemical analysis of burdock root constituents

An ethanolic solution of extracted burdock root was concentrated, suspended by water :and extracted by petroleum ether, chloroform, ethyl acetate and n-butanol. Then, the solution was purified by silica gel, Sephadex LH-20 and C-18 column chromatography to obtain 17 compounds. Nine structures of compounds, identified according to physical and chemical properties using IR, UV, ESIMS, H-1 NMR, C-13 NMR and other methods, include 3 petroleum ethers (beta-sitosterol, oleanolic acid, ursolic acid), 4 chloroforms (beta-sitosterol, daucosterol, syringaresinol, ethyl-beta-D-pyran fruit glycosides, 1 ethyl acetate (1,5-O-two caffeoylquinic acid) and n-butanol parts (succinic acid and 5-hydroxy maltol). Oleanolic acid, ursolic acid, clove-lignans, ethyl-beta-D-pyran fruit glycoside, succinic acid and 5-hydroxy maltol were first isolated from burdock.