Studies On The Binding Of Dna With The Inclusion Of Brilliant Green Inside The Cavity Of Gamma-Cyclodextrin

The interaction of brilliant green with herring sperm DNA was investigated in detail by spectrometric methods in gamma-cyclodextrin systems. On the condition of physiological pH, brilliant green prefers to form the 1:1 inclusion complex with gamma-cyclodextrin. All the evidences indicated that the binding modes between gamma-cyclodextrin-brilliant green and DNA were grooving binding and partial non-classical intercalative binding. The binding ratio of the inclusion complex with DNA is 6:1. The calculated thermodynamic parameters suggested that the binding of the inclusion complex to DNA was driven mainly by entropy.