Pseudomonas oryzae sp. nov. isolated from a paddy soil in South China

A Gram-staining-negative, rod-shaped and motile with several polar flagellums bacterium, designated WM-3 T , was isolated from a rice paddy soil in South China. Growth occurred with 0–3.0 % (w/v) NaCl (optimum 2.0 %), at pH 5.5–9.0 (optimum pH 7.0) and at 25–42 °C (optimum 30–37 °C) in liquid Reasoner’s 2A medium. Analysis of the 16S rRNA gene and gyr B gene sequences revealed that strain WM-3 T was most closely related to the type strains of the species Pseudomonas linyingensis and Pseudomonas sagittaria . Its sequence similarities with P. linyingensis CGMCC 1.10701 T and P. sagittaria JCM 18195 T were 97.4 and 97.3 %, respectively, for 16S rRNA gene, and were 94.1 and 94.2 %, respectively, for gyr B gene. DNA–DNA hybridization between strain WM-3 T and these two type strains showed relatedness of 35.6 and 30.9 %, respectively. G+C content of genomic DNA was 69.4 mol%. The whole-cell fatty acids mainly consisted of C 16:0 (30.0 %), C 16:1 ω 6c and/or C 16:1 ω 7c (19.3 %) and C 18:1 ω 6 c and/or C 18:1 ω 7 c (16.3 %). The results of phenotypic, chemotaxonomic and genotypic analyses clearly indicated that strain WM-3 T belongs to genus Pseudomonas but represents a novel species, for which the name Pseudomonas oryzae sp. nov. is proposed. The type strain is WM-3 T (=KCTC 32247 T =CGMCC 1.12417 T ).