The separation of flavonoids from Pongamia pinnata using combination columns in high-speed counter-current chromatography with a three-phase solvent system
Journal of Chromatography A(2013)
Abstract
•Preparing CCC combination columns using a new three-phase solvent system.•Tuning the retention times of solutes by varying the volume ratios of the binary stationary phases.•Successful application of CCC combination columns for the separation of natural products.
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Key words
High-speed counter-current chromatography (HSCCC),Three-phase solvent system,Combination column,Pongamia pinnata
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