尿酸钠结晶影响肾小管上皮细胞的紧密连接★
Chinese Journal of Tissue Engineering Research（2013）
Abstract
背景:目前研究来看,尿酸性结石与紧密连接蛋白及肾间质纤维化的关系仍未明确.目的:观察尿酸钠结晶对肾小管上皮细胞紧密连接的影响.方法:配制单钠尿酸钠晶体.将正常大鼠肾小管上皮细胞随机分为对照组和尿酸钠结晶组,分别用无血清培养基和尿酸钠结晶进行培养.免疫荧光和RT-PCR方法检测24,48,72 h紧密连接蛋白的表达.结果与结论:与对照组比较,尿酸钠结晶于不同时相刺激NRK-52E细胞后,紧密连接蛋白和mRNA表达下降(P<0.05),72 h时下降显著(P<0.01),蛋白表达出现重新分布现象.说明尿酸钠结晶可破坏肾小管上皮细胞紧密连接蛋白的结构、功能及导致分布异常.
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Key words
Claudin,Cytological experiments of tissue construction,Hyperuricemia,Membranous protein,Monosodium urate monohydrate crystals,NRK-52E cells,Rats,Renal interstitial fibrosis,Tight junctions,Tissue construction,Tissue construction photographs-containing paper,Transmembrane protein,Tubular epithelial cells
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