Mechanical compressive force inhibits adipogenesis of adipose stem cells.

ObjectiveThe aim of this study was to investigate effects of mechanical compressive force on differentiation of adipose-derived stem cells (ASCs) in vitro. Materials and methodsMice ASCs were treated with compressive force (2000 epsilon, 1Hz) for 2 or 6h after adipogenic induction for 3days, then oil red O staining was used to examine oil droplet-filled cells. Adipogenic genes, PPAR-1 and APN, were examined by real-time PCR and immunofluorescence (IF) staining was performed to test expression of de-PPAR- and ph-PPAR- at the protein level. ResultsOur data showed that mechanical compressive force reduced numbers of oil droplet-filled cells, and down-regulated mRNA levels of both PPAR-1 and APN and protein level of PPAR-, in ASCs. ConclusionsIn culture medium containing adipogenic stimuli, mechanical compressive force inhibited adipogenesis of ASCs.