Competitive immunoassay of progesterone by microchip electrophoresis with chemiluminescence detection.

•A novel MCE-CL based homogeneous competitive immunoassay method was developed for the determination of progesterone (P).•The assay was successfully used for the determination of P contents in serum human serum.•The MCE separation was accomplished within 80s with a detection limit of 3.8nM for P.•This method has not been reported, and can serve as an alternative tool for clinical assay of P.