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Analysis and solution of false-positives when testing CVA16 sera using an antibody assay against the EV71 virus.

Changbing Wang,Aiping You,Xingui Tian,Mingqi Zhao,Yi Chen,Tao Lin,Jianbin Zheng,Misi Xiao,Yingying Zhang,Lu Kuang,Zhenwen Zhou,Bing Zhu

Virus Research（2013）

Cited 6|Views35
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Abstract
•The VP3 is the cross-reacting antigen in the EV71 IgM-capture ELISA with CVA16 serum.•The VP1 detection method is as sensitive as the virus-antibody detection method.•The VP1 detection method can resolve the false-positive problem.
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Key words
HFMD,EV71,CVA16,ELISA,RT-PCR,CSF,FBS,HRP,TCID50,PBS,OD,SDS-PAGE,DAB,TMB
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