A bioengineered metastatic pancreatic tumor model for mechanistic investigation of chemotherapeutic drugs.

•We bioengineered a metastatic pancreatic tumor model with an electrospun scaffold.•The model featured an accelerated tumorigenesis and hepatic metastasis.•The model can be used to comparatively study chemotherapeutic drugs.•The model showed depleting CD133+CXCR4+ cells could prevent metastasis.•FOLFIRINOX is a preferred drug than gemcitabine in depleting CD133+CXCR4+ cells.