Understanding the pH-dependent immobilization efficacy of feruloyl esterase-C on mesoporous silica and its structure–activity changes

Christian Thörn,D.B.R.K. Gupta Udatha,Hao Zhou,Paul Christakopoulos,Evangelos Topakas,Lisbeth Olsson

Journal of Molecular Catalysis B: Enzymatic（2013）

引用 21|浏览3

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摘要

•Surface charge distribution affects enzyme orientation and substrate accessibility.•Immobilization pH alters the specific activity irrespective of reaction pH.•Enzyme product selectivity is buffer dependent.

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关键词

Enzyme immobilization,Feruloyl esterases,Mesoporous silica,Molecular simulations,Docking

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