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人脐血和外周血内皮祖细胞的分离培养和鉴定★◆

李欢欢,赵丽静,何旭,王娟,刘亢丁

Chinese Journal of Tissue Engineering Research（2013）

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Abstract
背景:国内外有关内皮祖细胞的分离培养和鉴定方面的文章很多,但是人脐血和外周血内皮祖细胞的鉴别方面文章不多.目的:从人脐血和外周血中分离出内皮祖细胞,并对其进行培养和鉴定.方法:选取密度梯度离心法从脐血和外周血中分离获得单个核细胞,按照1×106/cm2的浓度种植于预先铺有纤维连接蛋白的培养板中,用含血管内皮生长因子的M199培养基进行诱导培养.结果与结论:人脐血和外周血中存在内皮祖细胞,浓度梯度离心联合贴壁筛选获得的单个核细胞在血管内皮生长因子的诱导培养下可分化成内皮祖细胞,内皮祖细胞表达 CD34、CD133、CD105、KDR和 CD31,能吞噬乙酰化低密度脂蛋白,结合荆豆凝集素1,它们可作为体外分选内皮祖细胞的标志.
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Angiogenesis,Endothelial progenitor cells,Human umbilical cord blood,New endothelium,Peripheral blood,Stem cell culture and differentiation,Stem cells,Stem cells photographs-containing paper
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