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Evaluation of poly(ethylene glycol)/hydroxypropyl starch aqueous two-phase system for immunoglobulin G extraction

Qiang Wu,Dong-Qiang Lin,Shan-Jing Yao

Journal of Chromatography B（2013）

Cited 25|Views3
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Abstract
•Aqueous two-phase system was used to separate IgG from albumin containing feedstock.•Hydroxypropyl starch was used as the phase-forming polymer with relatively low cost.•NaCl addition and pH were optimized as key factors to improve the extraction of IgG.•High purification factor of IgG was obtained with two-step extraction process.
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Key words
Aqueous two-phase system,Hydroxypropyl starch,Human immunoglobulin G,Purification,Response surface methodology
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