Photochemical properties and phototoxicity of Pazufloxacin: a stable and transient study.
Journal of Photochemistry and Photobiology B: Biology(2013)
Abstract
Photochemical properties and phototoxicity of Pazufloxacin (PAX) were systematically investigated in aqueous solutions using UV–Vis, fluorescence, laser flash photolysis, pulse radiolysis and SDS–PAGE gel electrophoresis techniques. PAX triplet-state (3PAX∗) absorption spectra (λmax=570nm) was determined. 3PAX∗ was quenched by PAX and O2, with rate constants of 6.9×108 and 3.2×108dm3mol−1s−1, respectively. The pKa values (5.7 and 8.6) for the protonation equilibrium were determined by UV–Vis and fluorescence techniques. The PAX triplet energy (ET=260.3kJ/mol) was obtained using energy transfer method. The reaction of electron transfer from tryptophan (TrpH) and dGMP to 3PAX∗ was found with rate constants of 8.8×107 and 8.7×106dm3mol−1s−1, respectively. The rate constants for reactions of OH, SO4- and hydrated electron with PAX were found to be 5.8×108, 2.1×109 and 9×109dm3mol−1s−1, respectively. Based on the results obtained, a rational scheme for dGMP, TrpH and lysozyme photodamage induced by PAX was proposed.
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Key words
Pazufloxacin,Laser flash photolysis,Pulse radiolysis,Photooxidation,SDS–PAGE gel electrophoresis
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