油桐叶片愈伤组织诱导及植株再生

Zhiwu Shengli Xuebao/Plant Physiology Journal(2013)

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Abstract
以油桐无菌苗叶片为试材,研究不同种类及浓度的植物生长调节剂对愈伤组织诱导、分化、增殖及生根的影响.结果表明:叶片愈伤组织的最佳诱导培养基为1/2MS+2.0 mg·L-16-BA+1.0 mg·L-12,4-D,诱导率达100%;最佳分化培养基为1/2MS+3.0 mg·L-16-BA+0.1 mg·L-1 IBA+0.05 mg·L-1 IAA,分化率为86.36%;最佳继代增殖培养基为MS+3.0 mg·L-1 6-BA+0.05mg·L-1IBA;最佳生根培养基为1/2MS+0.1 mg·L-1 IBA,生根率93.83%.炼苗后移栽到泥炭土:珍珠岩:蛭石=2:1:1的基质中,成活率达92%以上.
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Key words
Adventitious shoots,Callus,Plant regeneration,Vernicia fordii
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