Transgenic expression of the functional fragment Hpa110-42 of the harpin protein hpa1 imparts enhanced resistance to powdery mildew in wheat
PLANT DISEASE(2014)
摘要
Powdery mildew, one of devastating diseases of wheat worldwide, is caused by Erysiphe graminis f. sp. tritici, a fungal species with constant population changes, which often poses challenges in disease management with host resistance. Transgenic approaches that utilize broad-spectrum resistance may limit changes of pathogen populations and contribute to effective control of the disease. The harpin protein Hpa1, produced by the rice bacterial blight pathogen, can induce resistance to bacterial blight and blast in rice. The fragment comprising residues 10 through 42 of Hpa1, Hpa1(10-42), is reportedly three- to eight-fold more effective than the full-length protein. This study evaluated the transgenic expression of the Hpa1(10-42) gene for resistance to powdery mildew in wheat caused by E. graminis f. sp. tritici. Nine Hpa1(10-42) transgenic wheat lines were generated. The genomic integration of Hpa1(10-42) was confirmed, and expression of the transgene was detected at different levels in the individual transgenic lines. Following inoculation with the E. graminis f. sp. tritici isolate Egt15 in the greenhouse, five transgenic lines had significantly higher levels of resistance to powdery mildew compared with nontransformed plants. Thus, transgenic expression of Hpa1(10-42) conferred resistance to one isolate of E. graminis f. sp. tritici in wheat in the greenhouse.
更多查看译文
关键词
Plant Growth-Promoting Bacteria
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要