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脉冲强光处理对杨梅多酚氧化酶活性的影响

王正东,戚向阳,曹少谦,袁勇军

Journal of Chinese Institute of Food Science and Technology（2013）

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Abstract
采用响应面法研究脉冲强光对杨梅中多酚氧化酶(ppO)活性的影响.设定3个影响因素分别为脉冲强度(150～450 mJ/cm2),闪照次数(3～7),酶溶液pH(3.0～7.0).实验结果表明:各处理因素对酶的活性具有显著性的影响(p＜0.05),其中溶液pH值对酶活性的影响最为显著.该回归方程的拟合度高,能够预测不同处理条件对酶的钝化效果.根据回归方程可知,在pH 3.2的条件下,采用450 mJ/cm2闪照7次的脉冲强光处理,可使PPO酶的钝化率达到54.7％.
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Key words
Polyphenol oxidase,Pulsed Light,Response surface
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