异养硝化-好氧反硝化菌HN-02的筛选及其特性
Chinese Journal of Applied and Environmental Biology（2013）
Abstract
为了向生物强化处理高浓度含氮废水提供高效菌株,从活性污泥中筛选到一株效果较好的异养硝化-好氧反硝化菌,命名为HN-02.形态观察、生理生化鉴定及16S rDNA序列分析表明,该菌株为短杆状革兰氏阴性菌,并鉴定为气单胞菌属(Aeromonas),且能扩增出相关好氧反硝化基因napA.其最佳碳源为琥珀酸,其次是蔗糖和柠檬酸钠；最佳生长温度为30℃；最佳pH范围为6-9.在好氧条件下,该菌能独立降解多种形态的氮,以氨氮、亚硝氮、硝氮为唯一氮源时,24 h总氮去除率分别为77.71％、53.34％、56.80％.经定性验证,脱氮气体产物含有氮氧化物.
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Key words
Aeromonas,Bioaugmentation,Heterotrophic nitrification - Aerobic denitrification,Nitrogen removal,Strain growth characteristics
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